Rapid and Specific Detection of the O15:K52:H1 Clonal Group of Escherichia coli by Gene-Specific PCR-Reference-Cited by-同舟云学术

Rapid and Specific Detection of the O15:K52:H1 Clonal Group of Escherichia coli by Gene-Specific PCR

Published:2004-08 Issue:8 Volume:42 Page:3841-3843
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Johnson James R.¹²,Owens Krista¹²,Sabate Montse³,Prats Guillem³

Affiliation:

1. Mucosal and Vaccine Research Center, Minneapolis VA Medical Center

2. Department of Medicine, University of Minnesota, Minneapolis, Minnesota

3. Clinical Microbiology Laboratory, Val d'Hebron Hospital, Barcelona, Spain

Abstract

ABSTRACT Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC , i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.8.3841-3843.2004

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