Affiliation:
1. Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111,1 and
2. Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts 021182
Abstract
ABSTRACT
Synthesis of glutamate, the cell's major donor of nitrogen groups and principal anion, occupies a significant fraction of bacterial metabolism. In
Bacillus subtilis
, the
gltAB
operon, encoding glutamate synthase, requires a specific positive regulator, GltC, for its expression. In addition, the
gltAB
operon was shown to be repressed by TnrA, a regulator of several other genes of nitrogen metabolism and active under conditions of ammonium (nitrogen) limitation. TnrA was found to bind directly to a site immediately downstream of the
gltAB
promoter. As is true for other genes, the activity of TnrA at the
gltAB
promoter was antagonized by glutamine synthetase under certain growth conditions.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
76 articles.
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