Affiliation:
1. Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et Microbiologie, Centre Nationale de la Recherche Scientifique, 13402 Marseille Cedex 20, France
Abstract
ABSTRACT
The
bisZ
gene of
Escherichia coli
was previously described as encoding a minor biotin sulfoxide (BSO) reductase in addition to the main cytoplasmic BSO reductase, BisC. In this study,
bisZ
has been renamed
torZ
based on the findings that (i) the
torZ
gene product, TorZ, is able to reduce trimethylamine
N
-oxide (TMAO) more efficiently than BSO; (ii) although TorZ is more homologous to BisC than to the TMAO reductase TorA (63 and 42% identity, respectively), it is located mainly in the periplasm as is TorA; (iii)
torZ
belongs to the
torYZ
operon, and the first gene,
torY
(formerly
yecK
), encodes a pentahemic
c
-type cytochrome homologous to the TorC cytochrome of the TorCAD respiratory system. Furthermore, the
torYZ
operon encodes a third TMAO respiratory system, with catalytic properties that are clearly different from those of the TorCAD and the DmsABC systems. The
torYZ
and the
torCAD
operons may have diverged from a common ancestor, but, surprisingly, no
torD
homologue is found in the sequences around
torYZ
. Moreover, the
torYZ
operon is expressed at very low levels under the conditions tested, and, in contrast to
torCAD
, it is not induced by TMAO or dimethyl sulfoxide.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
56 articles.
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