Improved Method for Detection of Starch Hydrolysis-Reference-Cited by-同舟云学术

Improved Method for Detection of Starch Hydrolysis

Published:1982-09 Issue:3 Volume:44 Page:747-750
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Dhawale Motiram R.¹,Wilson J. Jeffrey¹,Khachatourians George G.¹,Ingledew W. Mike¹

Affiliation:

1. Agricultural Microbiology Section, Dairy and Food Science Department, University of Saskatchewan, Saskatoon, Canada, S7N 0W0

Abstract

A new starch hydrolysis detection method which does not rely on iodine staining or the use of color-complexed starch is described. A linear relationship was obtained with agar-starch plates when net clearing zones around colonies of yeasts were plotted against enzyme levels (semilogarithm scale) produced by the same yeast strains in liquid medium. A similar relationship between starch clearing zones and α-amylase levels from three different sources was observed. These observations suggest that the method is useful in mutant isolations, strain improvement programs, and the prediction of α-amylase activities in culture filtrates or column effluents.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.44.3.747-750.1982

Reference18 articles.

1. Enzymatic catalysis in solidified media;Ceska M.;Eur. J. Biochem.,1971

2. Hydrolysis of a water-insoluble substrate incorporated into solidified medium by the enzyme aamylase contained in normal blood, serum and plasma;Ceska M.;Clin. Chim. Acta,1972

3. Hydrolysis of a water insoluble substrate incorporated into solidified agar medium by enzyme aamylase contained in serum and urine of patients suffering from acute pancreatitis;Ceska M.;Clin. Chim. Acta,1972

4. A new method for determination of a-amylase;Ceska M.;Experientia,1969

5. A method for the detection of starch hydrolysis by bacteria;Iverson W. G.;J. Appl. Bacteriol.,1974

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