Significant Finding of Bordetella holmesii DNA in Nasopharyngeal Samples from French Patients with Suspected Pertussis

Author:

Njamkepo Elisabeth12,Bonacorsi Stéphane3,Debruyne Monique4,Gibaud Sophie Anne5,Guillot Sophie12,Guiso Nicole12

Affiliation:

1. Institut Pasteur, Molecular Prevention and Therapy of Human Diseases, National Centre of Reference of Whooping Cough and Other Bordetelloses

2. CNRS-URA 3012, Paris, France

3. Hôpital Robert Debré, Service de Microbiologie, Paris, France

4. Laboratoire Cerba, Saint Ouen L'Aumône, France

5. Laboratoire de Bactériologie, Centre Hospitalier Universitaire de Nantes, Nantes, France

Abstract

ABSTRACT Pertussis is routinely diagnosed with real-time PCR based on insertion sequence IS 481 , which is not specific for Bordetella pertussis . We conducted a retrospective study using real-time PCRs specific for Bordetella pertussis and for Bordetella holmesii on 177 samples positive for IS 481 PCR. Bordetella holmesii DNA was detected in 20.3% samples collected from adolescents and adults.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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