Novel Duplex Real-Time PCR Assay Detects Bordetella holmesii in Specimens from Patients with Pertussis-Like Symptoms in Ontario, Canada-Reference-Cited by-同舟云学术

Novel Duplex Real-Time PCR Assay Detects Bordetella holmesii in Specimens from Patients with Pertussis-Like Symptoms in Ontario, Canada

Published:2010-04 Issue:4 Volume:48 Page:1435-1437
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Guthrie J. L.¹,Robertson A. V.²,Tang P.¹,Jamieson F.¹³,Drews S. J.⁴⁵

Affiliation:

1. Ontario Agency for Health Protection and Promotion, Toronto, Ontario, Canada

2. Applied Biosystems, Foster City, California

3. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada

4. ProvLab, Calgary, Alberta, Canada

5. Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada

Abstract

ABSTRACT Bordetella holmesii is a human pathogen found mainly in immunocompromised patients. A specific real-time PCR assay was developed and successfully used to identify specimens from which B. holmesii was misidentified as Bordetella pertussis and to establish the prevalence of B. holmesii in Ontario patients with pertussis-like symptoms.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02417-09

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