Isolation and Identification of Psychrophilic Species of Clostridium from Milk

Author:

Bhadsavle C. H.1,Shehata T. E.1,Collins E. B.1

Affiliation:

1. Department of Food Science and Technology, University of California, Davis, California 95616

Abstract

Four of 48 raw milk samples contained catalase-negative, gram-positive, motile, sporeforming, rod-shaped bacteria that grew optimally at 22 to 30 C and slowly at low temperatures. Isolates from two samples had a minimal growth temperature of 4 C, were anaerobic, and had characteristics similar to Clostridium hastiforme ; those from the other two samples had a minimal growth temperature of 0 � 1 C, were anaerobic, aerotolerant, and had characteristics similar to C. carnis . Specific growth rates, doubling times, ability to grow in pasteurized milk stored in commercial cartons, and resistance of spores to heating were determined for one strain of C. hastiforme .

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference20 articles.

1. Psychrotrophic clostridia;Beerens H.;J. Appl. Bacteriol.,1965

2. Breed R S. E. G. D. Murray and N. R Smith. 1957. Bergey's manual of determinative bacteriology 7th ed. The Williams & Wilkins Co. Baltimore.

3. Foster E. M. F. E. Nelson M. L. Speck R. N. Doetsch and J. C. Olson Jr. 1957. Dairy microbiology. Prentice-Hall Englewood Cliffs N.J.

4. Fredette V. 1960. Manual of methods for the selective isolation rapid identification and antibiogrammetry of the anaerobic bacteria. Dept. of Microbiol. and Hygiene Univ. of Montreal Montreal Canada.

5. Anaerobic bacteria in dairy products. H. Relationship of anaerobic bacteria to certain abnormal fermentations;Hussong R V;J. Bacteriol.,1930

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