Detection and Confirmation of Clostridium botulinum in Water Used for Cooling at a Plant Producing Low-Acid Canned Foods-Reference-Cited by-同舟云学术

Detection and Confirmation of Clostridium botulinum in Water Used for Cooling at a Plant Producing Low-Acid Canned Foods

Published:2010-11-15 Issue:22 Volume:76 Page:7653-7657
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Sachdeva Amita¹,Defibaugh-Chávez Stephanie L. H.¹,Day James B.¹,Zink Donald¹,Sharma Shashi K.¹

Affiliation:

1. U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, Maryland 20740

Abstract

ABSTRACT Our laboratory tested water samples used for cooling low-acid canned foods at a canning facility under investigation by the U.S. Food and Drug Administration. We used an enzyme-linked immunosorbent assay with digoxigenin-labeled antibodies (DIG-ELISA) and real-time PCR as screening methods and confirmed the presence of neurotoxin-producing Clostridium botulinum in the samples by mouse bioassay.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00820-10

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