Pulsed-Field Gel Electrophoresis Profile Changes Resulting from Spontaneous Chromosomal Deletions in Enterohemorrhagic Escherichia coli O157:H7 during Passage in Cattle-Reference-Cited by-同舟云学术

Pulsed-Field Gel Electrophoresis Profile Changes Resulting from Spontaneous Chromosomal Deletions in Enterohemorrhagic Escherichia coli O157:H7 during Passage in Cattle

Published:2009-09 Issue:17 Volume:75 Page:5719-5726
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Yoshii Noriyo¹,Ogura Yoshitoshi²,Hayashi Tetsuya²,Ajiro Takashi³,Sameshima Toshiya⁴,Nakazawa Muneo⁵,Kusumoto Masahiro¹,Iwata Taketoshi¹,Akiba Masato¹

Affiliation:

1. Safety Research Team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba 305-0856, Japan

2. Division of Microbial Diseases, Department of Infectious Diseases, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan

3. Division of Livestock Industry, Department of Agriculture, Forestry and Fisheries, Miyagi Prefectural Government, 3-8-1 Honcho, Aoba-ku, Sendai, Miyagi 980-8570, Japan

4. Center for Animal Disease Control and Prevention, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan

5. Epidemiological Research Team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan

Abstract

ABSTRACT A total of 905 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates that were recovered from experimentally infected cattle, in addition to the inoculated strain, were analyzed by pulsed-field gel electrophoresis (PFGE). Twelve PFGE profiles other than that of the inoculated strain were observed. We successfully identified five distinct chromosomal deletions that affected the PFGE profiles using whole-genome PCR scanning and DNA sequencing analysis. The changes in PFGE profiles of EHEC O157:H7 isolates after passage through the intestinal tract of cattle were partially generated by deletion of chromosomal regions.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00558-09

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