Determining UV Inactivation of Toxoplasma gondii Oocysts by Using Cell Culture and a Mouse Bioassay-Reference-Cited by-同舟云学术

Determining UV Inactivation of Toxoplasma gondii Oocysts by Using Cell Culture and a Mouse Bioassay

Published:2010-08 Issue:15 Volume:76 Page:5140-5147
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Ware Michael W.¹,Augustine Swinburne A. J.¹,Erisman David O.¹,See Mary Jean²,Wymer Larry¹,Hayes Samuel L.³,Dubey J. P.⁴,Villegas Eric N.¹²

Affiliation:

1. National Exposure Research Laboratory

2. Department of Biological Sciences, McMicken College of Arts and Sciences, University of Cincinnati, Cincinnati, Ohio 45220

3. National Risk Management Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268

4. Animal Parasitic Disease Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705

Abstract

ABSTRACT The effect of UV exposure on Toxoplasma gondii oocysts has not been completely defined for use in water disinfection. This study evaluated UV-irradiated oocysts by three assays: a SCID mouse bioassay, an in vitro T. gondii oocyst plaque (TOP) assay, and a quantitative reverse transcriptase real-time PCR (RT-qPCR) assay. The results from the animal bioassay show that 1- and 3-log 10 inactivation is achieved with 4 mJ/cm 2 UV and 10 mJ/cm 2 low-pressure UV, respectively. TOP assay results, but not RT-qPCR results, correlate well with bioassay results. In conclusion, a 3-log 10 inactivation of T. gondii oocysts is achieved by 10-mJ/cm 2 low-pressure UV, and the in vitro TOP assay is a promising alternative to the mouse bioassay.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00153-10

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