An Endogenous Staphylococcus aureus CRISPR-Cas System Limits Phage Proliferation and Is Efficiently Excised from the Genome as Part of the SCC <i>mec</i> Cassette-Reference-Cited by-同舟云学术

An Endogenous Staphylococcus aureus CRISPR-Cas System Limits Phage Proliferation and Is Efficiently Excised from the Genome as Part of the SCC mec Cassette

Published:2023-08-17 Issue:4 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Mikkelsen Kasper¹^ORCID,Bowring Janine Zara¹^ORCID,Ng Yong Kai¹²,Svanberg Frisinger Frida¹,Maglegaard Julie Kjærsgaard¹,Li Qiuchun³^ORCID,Sieber Raphael N.²^ORCID,Petersen Andreas²,Andersen Paal Skytt¹²,Rostøl Jakob T.⁴,Høyland-Kroghsbo Nina Molin¹⁵^ORCID,Ingmer Hanne¹^ORCID

Affiliation:

1. Department of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark

2. Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark

3. Jiangsu Key Lab of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China

4. Centre for Bacterial Resistance Biology, Imperial College London, London, United Kingdom

5. Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark

Abstract

CRISPR-Cas is an adaptive immune system protecting bacteria and archaea against mobile genetic elements such as phages. In strains of Staphylococcus aureus , CRISPR-Cas is rare, but when present, it is located within the SCC mec element, which encodes resistance to methicillin and other β-lactam antibiotics.

Funder

H. Lundbeck A/S

Danmarks Frie Forskningsfond

European Molecular Biology Organization

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01277-23

Reference62 articles.

1. CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

2. CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

3. Molecular mechanisms of CRISPR–Cas spacer acquisition

4. The Biology of CRISPR-Cas: Backward and Forward

5. Evolution and classification of the CRISPR–Cas systems