High-Throughput Multiplex SARS-CoV-2 IgG Microsphere Immunoassay for Dried Blood Spots: A Public Health Strategy for Enhanced Serosurvey Capacity

Author:

Styer Linda M.12,Hoen Rebecca3,Rock Jean1,Yauney Erica1,Nemeth Katherine1,Bievenue Rachel1,Parker Monica M.1ORCID

Affiliation:

1. Wadsworth Center, New York State Department of Health, Albany, New York, USA

2. Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, New York, USA

3. Bureau of Surveillance and Data Systems, New York State Department of Health, Albany, New York, USA

Abstract

Testing for antibodies to SARS-CoV-2 has been used to estimate the prevalence of COVID-19 in different populations. Seroprevalence studies, or serosurveys, were especially useful during the early phase of the pandemic when diagnostic testing was not widely available, and the resulting seroprevalence estimates played an important role in public health decision making.

Funder

HHS | Centers for Disease Control and Prevention

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Reference22 articles.

1. Gronvall G, Connell N, Farley JE, Inglesby T, Jennings JM, Mehta SH, West R, Kobokovich A. 2020. Developing a national strategy for SARS-CoV-2 serosurveys in the United States. Johns Hopkins Center for Health Security, Baltimore, MD. https://www.centerforhealthsecurity.org/our-work/publications/developing-a-national-strategy-for-sars-cov-2-serosurveys-in-the-united-states. Accessed 25 April 2021.

2. Development and validation of assay protocols for use with dried blood spot samples

3. What a drop can do: Dried blood spots as a minimally invasive method for integrating biomarkers into population-based research

4. Centers for Disease Control and Prevention. 2017. Shipping guidelines for dried-blood spot specimens. https://www.cdc.gov/labstandards//pdf/nsqap/Bloodspot_Transportation_Guidelines.pdf. Accessed April 25 2021.

5. A serological assay to detect SARS-CoV-2 antibodies in at-home collected finger-prick dried blood spots

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