Development of a novel serological assay for the detection of mpox infection in vaccinated populations-Reference-Cited by-同舟云学术

Development of a novel serological assay for the detection of mpox infection in vaccinated populations

Published:2023-10 Issue:10 Volume:95 Page:
ISSN:0146-6615
Container-title:Journal of Medical Virology
language:en
Short-container-title:Journal of Medical Virology

Author:

Yates Jennifer L.¹²^ORCID,Hunt Danielle T.¹,Kulas Karen E.¹,Chave Karen J.³,Styer Linda¹²,Chakravarthi Sandhya T.³,Cai Gianna Y.⁴⁵,Bermúdez‐González Maria C.⁴⁵,Kleiner Giulio⁴⁵,Altman Deena⁶^ORCID,Srivastava Komal⁴⁵,Simon Viviana⁴⁵⁶⁷⁸,Feihel Dennis⁹¹⁰,McGowan Joseph⁹¹⁰,Hogrefe Wayne¹¹,Noone Philip¹¹,Egan Christina¹²,Slifka Mark K.¹²¹³,Lee William T.¹²,

Affiliation:

1. Division of Infectious Diseases, Wadsworth Center New York State Department of Health Albany New York USA

2. Department of Biomedical Sciences The School of Public Heath, The University at Albany Albany New York USA

3. Scientific Cores, Wadsworth Center New York State Department of Health Albany New York USA

4. Center for Vaccine Research and Pandemic Preparedness Icahn School of Medicine at Mount Sinai New York USA

5. Department of Microbiology Icahn School of Medicine at Mount Sinai New York USA

6. Division of Infectious Diseases, Department of Medicine Icahn School of Medicine at Mount Sinai New York USA

7. Department of Pathology Molecular and Cell Based Medicine, Icahn School of Medicine at Mount Sinai New York USA

8. The Global Health and Emerging Pathogens Institute Icahn School of Medicine at Mount Sinai New York USA

9. Department of Medicine North Shore University Hospital Manhasset New York USA

10. Zucker School of Medicine at Hofstra/Northwell Hempstead New York USA

11. Aalto Bio Reagents Dublin Ireland

12. Najit Technologies, Inc. Beaverton Oregon USA

13. Oregon National Primate Research Center Oregon Health and Science University Beaverton Oregon USA

Abstract

AbstractIn 2022 the World Health Organization declared a Public Health Emergency for an outbreak of mpox, the zoonotic Orthopoxvirus (OPV) affecting at least 104 nonendemic locations worldwide. Serologic detection of mpox infection is problematic, however, due to considerable antigenic and serologic cross‐reactivity among OPVs and smallpox‐vaccinated individuals. In this report, we developed a high‐throughput multiplex microsphere immunoassay using a combination of mpox‐specific peptides and cross‐reactive OPV proteins that results in the specific serologic detection of mpox infection with 93% sensitivity and 98% specificity. The New York State Non‐Vaccinia Orthopoxvirus Microsphere Immunoassay is an important tool to detect subclinical mpox infection and understand the extent of mpox spread in the community through retrospective analysis.

Funder

National Institutes of Health

Publisher

Wiley

Subject

Infectious Diseases,Virology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/jmv.29134

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