Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells-Reference-Cited by-同舟云学术

Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells

Published:2022-08-31 Issue:4 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Shi Fang-Shu¹,Yu Yin²,Li Ya-Li¹,Cui Lilan³,Zhao Zhuangzhuang¹,Wang Mi³,Wang Bin¹,Zhang Rong²,Huang Yao-Wei¹⁴^ORCID

Affiliation:

1. Department of Veterinary Medicine, Zhejiang University, Hangzhou, China

2. Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, China

3. Novoprotein Scientific Inc., Shanghai, China

4. Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China

Abstract

The intracellular localization of SARS-CoV-2 replicase nonstructural proteins (nsp) during infection has not been fully elucidated. In this study, we systematically analyzed the expression and subcellular localization of 11 distinct viral nsp and dsRNA over time in SARS-CoV-2-infected cells by using individual antibody against these replicase proteins.

Funder

Key Research and development program of Zhejiang province

National Natural Science Foundation of China

MOST | National Key Research and Development Program of China

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00744-22

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