Mapping Molecular Recognition of β1,3-1,4-Glucans by a Surface Glycan-Binding Protein from the Human Gut Symbiont Bacteroides ovatus-Reference-Cited by-同舟云学术

Mapping Molecular Recognition of β1,3-1,4-Glucans by a Surface Glycan-Binding Protein from the Human Gut Symbiont Bacteroides ovatus

Published:2021-12-22 Issue:3 Volume:9 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Correia Viviana G.¹²^ORCID,Trovão Filipa¹²^ORCID,Pinheiro Benedita A.¹²^ORCID,Brás Joana L. A.³,Silva Lisete M.⁴^ORCID,Nunes Cláudia⁵^ORCID,Coimbra Manuel A.⁶^ORCID,Liu Yan⁴,Feizi Ten⁴,Fontes Carlos M. G. A.³⁷,Mulloy Barbara⁴,Chai Wengang⁴^ORCID,Carvalho Ana Luísa¹²^ORCID,Palma Angelina S.¹²^ORCID

Affiliation:

1. UCIBIO, Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, Caparica, Portugal

2. Associate Laboratory i4HB—Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, Caparica, Portugal

3. NZYTech Genes & Enzymes, Lisbon, Portugal

4. Glycosciences Laboratory, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, United Kingdom

5. CICECO, Department of Chemistry, University of Aveiro, Aveiro, Portugal

6. LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Aveiro, Portugal

7. CIISA, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal

Abstract

With the knowledge of bacterial gene systems encoding proteins that target dietary carbohydrates as a source of nutrients and their importance for human health, major efforts are being made to understand carbohydrate recognition by various commensal bacteria. Here, we describe an integrative strategy that combines carbohydrate microarray technology with structural studies to further elucidate the molecular determinants of carbohydrate recognition by BoSGBP MLG -A, a key protein expressed at the surface of Bacteroides ovatus for utilization of mixed-linkage β1,3-1,4-glucans.

Funder

Associate Laboratory LAQV-REQUIMTE

CICECO-Aveiro Institute of Materials

Applied Molecular Biosciences Unit

MEC | Fundação para a Ciência e a Tecnologia

March of Dimes Foundation

Wellcome

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

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