Portable Differential Detection of CTX-M ESBL Gene Variants, bla CTX-M-1 and bla CTX-M-15 , from Escherichia coli Isolates and Animal Fecal Samples Using Loop-Primer Endonuclease Cleavage Loop-Mediated Isothermal Amplification-Reference-Cited by-同舟云学术

Portable Differential Detection of CTX-M ESBL Gene Variants, bla _CTX-M-1 and bla _CTX-M-15 , from Escherichia coli Isolates and Animal Fecal Samples Using Loop-Primer Endonuclease Cleavage Loop-Mediated Isothermal Amplification

Published:2023-02-14 Issue:1 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Higgins Owen¹²^ORCID,Chueiri Alexandra³²,O'Connor Louise³²,Lahiff Sinéad¹²,Burke Liam³²,Morris Dearbhaile³²,Pfeifer Nicola Maria⁴,Santamarina Belén González⁴,Berens Christian⁴,Menge Christian⁴,Caniça Manuela⁵,Manageiro Vera⁵^ORCID,Kisand Veljo⁶,Hassan Marwa M.⁷,Gardner Brian⁷,van Vliet Arnoud H. M.⁷^ORCID,La Ragione Roberto M.⁷⁸,Gonzalez-Zorn Bruno⁹^ORCID,Smith Terry J.¹²

Affiliation:

1. Molecular Diagnostics Research Group, School of Biological and Chemical Sciences, University of Galway, Galway, Ireland

2. Centre for One Health, Ryan Institute, University of Galway, Galway, Ireland

3. Antimicrobial Resistance and Microbial Ecology Group, School of Medicine, University of Galway, Galway, Ireland

4. Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Molecular Pathogenesis, Jena, Germany

5. National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal

6. Institute of Technology, University of Tartu, Tartu, Estonia

7. Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, United Kingdom

8. Department of Microbial Sciences, School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, United Kingdom

9. Antimicrobial Resistance Unit, Veterinary School and VISAVET, Complutense University of Madrid, Spain

Abstract

CTX-M ESBL-producing E. coli is an increasing AMR public health issue with the transmission between animals and humans via zoonotic pathogens now a major area of interest. Accurate and timely identification of ESBL-expressing E. coli CTX-M variants is essential for disease monitoring, targeted antibiotic treatment and infection control.

Funder

European Union Horizon 2020 Research and Innovation Programme

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.03316-22

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