Development of a Multiplex Real-Time PCR Assay for Mycobacterium bovis BCG and Validation in a Clinical Laboratory-Reference-Cited by-同舟云学术

Development of a Multiplex Real-Time PCR Assay for Mycobacterium bovis BCG and Validation in a Clinical Laboratory

Published:2021-10-31 Issue:2 Volume:9 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Duffy Shannon C.¹²^ORCID,Venkatesan Manigandan³,Chothe Shubhada⁴,Poojary Indira⁵,Verghese Valsan Philip⁶,Kapur Vivek⁴⁵,Behr Marcel A.¹²⁷^ORCID,Michael Joy Sarojini³

Affiliation:

1. Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada

2. McGill International TB Centre, Montreal, Quebec, Canada

3. Department of Clinical Microbiology, Christian Medical College Vellore, Vellore, Tamil Nadu, India

4. Department of Animal Science, The Pennsylvania State University, University Park, Pennsylvania, USA

5. Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, Pennsylvania, USA

6. Department of Pediatric Infectious Diseases, Christian Medical College Vellore, Vellore, Tamil, India

7. Department of Medicine, McGill University, Montreal, Quebec, Canada

Abstract

Vaccination against tuberculosis with bacillus Calmette-Guérin (BCG) can lead to adverse events, including a rare but life-threatening complication of disseminated BCG. This complication often occurs in young children with immunodeficiencies and is associated with an ∼60% mortality rate.

Funder

Gouvernement du Canada | Canadian Institutes of Health Research

Bill and Melinda Gates Foundation

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/Spectrum.01098-21

Reference23 articles.

1. World Health Organization. 2020. Global tuberculosis report 2020. World Health Organization, Geneva, Switzerland.

2. World Health Organization. 2012. Information sheet observed rate of vaccine reactions Bacille Calmette-Guérin (BCG) vaccine. World Health Organization Geneva Switzerland.

3. Disseminated Bacillus Calmette-Guérin and susceptibility to mycobacterial infection—implications on Bacillus Calmette-Guérin vaccinations;Lee PPW;Ann Acad Med Singap,2015

4. Bacillus Calmette-Guérin (BCG) complications associated with primary immunodeficiency diseases

5. Disseminated bacille Calmette-Guerin disease in HIV-infected South African infants

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