Shiga Toxin (Stx) Phage-Encoded Lytic Genes Are Not Required for the Mouse Virulence of O157:H7 Escherichia coli Stx2-Producing Clinical Isolates-Reference-Cited by-同舟云学术

Shiga Toxin (Stx) Phage-Encoded Lytic Genes Are Not Required for the Mouse Virulence of O157:H7 Escherichia coli Stx2-Producing Clinical Isolates

Published:2023-06-15 Issue:3 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Atitkar R. R.¹²^ORCID,Hauser J. R.¹²,Melton-Celsa A. R.¹

Affiliation:

1. Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA

2. Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, Maryland, USA

Abstract

The release of Stx from STEC has been thought to be tied to phage-mediated lysis of the host bacterial cell. In this study, we found that the stx 2a phage lytic genes are not required for the virulence of pathogenic O157:H7 clinical isolates in a murine model of STEC infection or for release of Stx2a into the supernatant of bacterial cultures. These results point to an alternate mechanism for Stx2a release from STEC strains.

Funder

HHS | National Institutes of Health

US | Uniformed Services University of the Health Sciences

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00372-23

Reference22 articles.

1. Escherichia coli: a brief review of diarrheagenic pathotypes and their role in diarrheal diseases in Iran;Jafari A;Iran J Microbiol,2012

2. Foodborne Illness Acquired in the United States—Major Pathogens

3. Centers for Disease Control and Prevention. 2021. National enteric disease surveillance: Shiga toxin-producing E. coli (STEC) annual report 2017.

4. Shiga Toxin Producing Escherichia coli

5. Long-term outcomes of Shiga toxin hemolytic uremic syndrome