MinION Sequencing of Yeast Mock Communities To Assess the Effect of Databases and ITS-LSU Markers on the Reliability of Metabarcoding Analysis-Reference-Cited by-同舟云学术

MinION Sequencing of Yeast Mock Communities To Assess the Effect of Databases and ITS-LSU Markers on the Reliability of Metabarcoding Analysis

Published:2023-02-14 Issue:1 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Conti Angela¹,Casagrande Pierantoni Debora¹,Robert Vincent²,Corte Laura¹³,Cardinali Gianluigi¹³^ORCID

Affiliation:

1. Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy

2. Westerdjik Institute for Biodiversity, Utrecht, Netherlands

3. CEMIN Excellence Research Centre, Perugia, Italy

Abstract

Metagenomic analyses are fundamental in many research areas; therefore, improvement of methods and protocols for the description of microbial communities becomes more and more necessary. Long-read sequencing could be used for reducing biases due to the multicopy nature of rDNA sequences and short-read limitations.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01052-22

Reference52 articles.

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