LI-Detector: a Method for Curating Ordered Gene-Replacement Libraries-Reference-Cited by-同舟云学术

LI-Detector: a Method for Curating Ordered Gene-Replacement Libraries

Published:2022-08-31 Issue:4 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Goodall Emily C. A.¹^ORCID,Morris Faye C.²^ORCID,McKeand Samantha A.²,Sullivan Rudi²,Warner Isabel A.¹^ORCID,Sheehan Emma²,Boelter Gabriela²,Icke Christopher¹,Cunningham Adam F.³^ORCID,Cole Jeffrey A.²,Banzhaf Manuel²,Bryant Jack A.²,Henderson Ian R.¹^ORCID

Affiliation:

1. Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia

2. Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom

3. Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, United Kingdom

Abstract

The construction of ordered gene replacement libraries requires significant investment of time and resources to create a valuable community resource. During construction, technical errors may result in a limited number of incorrect mutants being made. Such mutants may confound the output of subsequent experiments. Here, using the remarkable E. coli Keio knockout library, we describe a method to rapidly validate the construction of every mutant.

Funder

UKRI | Biotechnology and Biological Sciences Research Council

UKRI | Medical Research Council

Wellcome Trust

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00833-22

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