Optimized Replication of Arrayed Bacterial Mutant Libraries Increases Access to Biological Resources-Reference-Cited by-同舟云学术

Optimized Replication of Arrayed Bacterial Mutant Libraries Increases Access to Biological Resources

Published:2023-08-17 Issue:4 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Willett Julia L. E.¹^ORCID,Barnes Aaron M. T.¹,Brunson Debra N.²,Lecomte Alexandre³,Robertson Ethan B.¹,Dunny Gary M.¹

Affiliation:

1. Department of Microbiology and Immunology, University of Minnesota Medical School, Minneapolis, Minnesota, USA

2. Department of Oral Biology, University of Florida College of Dentistry, Gainesville, Florida, USA

3. Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France

Abstract

Arrayed mutant collections drive robust genetic screens, but few protocols exist for replication of these resources and subsequent quality control. Increasing the distribution of arrayed biological collections will increase the accessibility and use of these resources. Developing standardized techniques for replication of these resources is essential for ensuring their quality and usefulness to the scientific community.

Funder

France 2030

The Mica division of INRAE de Jouy-en-Josas

American Heart Association

Boehringer Ingelheim Fonds

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01693-23