Rapid Identification of Drug Resistance and Phylogeny in M. tuberculosis, Directly from Sputum Samples

Author:

Barbosa-Amezcua Martín1,Cuevas-Córdoba Betzaida12,Fresno Cristóbal34,Haase-Hernández Joshua I.3,Carrillo-Sánchez Karol5,Mata-Rocha Minerva6,Muñoz-Torrico Marcela7,Bäcker Claudia8,González-Covarrubias Vanessa1,Alaez-Verson Carmen5,Soberón Xavier19ORCID

Affiliation:

1. Laboratorio de Farmacogenómica, Instituto Nacional de Medicina Genómica (INMEGEN), Mexico City, México

2. Instituto de Investigaciones Biológicas, Universidad Veracruzana, Xalapa, Veracruz, México

3. Departamento de Desarrollo Tecnológico, Instituto Nacional de Medicina Genómica (INMEGEN), Mexico City, México

4. Centro de Investigación en Ciencias de la Salud (CICSA), Universidad Anáhuac México, Naucalpan de Juárez, México

5. Laboratorio de Diagnóstico Genómico, Instituto Nacional de Medicina Genómica (INMEGEN), Mexico City, México

6. Unidad de Investigación Médica en Epidemiología Clínica, UMAE Hospital de Pediatría, Centro Médico Nacional “Siglo XXI”, Instituto Mexicano del Seguro Social (IMSS), Mexico City, México

7. Clínica de Tuberculosis y Enfermedades Pleurales, Instituto Nacional de Enfermedades Respiratorias (INER), Mexico City, México

8. Laboratorio de Micobacterias, Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE), Secretaría de Salud, Mexico City, México

9. Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Cuernavaca, Morelos, México

Abstract

The COVID-19 pandemic negatively affected the progress in accessing essential Tuberculosis (TB) services and reducing the burden of TB disease, resulting in a decreased detection of new cases and increased deaths. Generating molecular diagnostic tests with faster results without losing reliability is considered a priority.

Funder

Consejo Nacional de Ciencia y Tecnología

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Reference55 articles.

1. World Health Organization. 2021. Global tuberculosis report. World Health Organization, Geneva, Switzerland. https://www.who.int/publications/i/item/9789240037021.

2. O’Neill J. 2019. Tackling drug-resistant infections globally: final report and recommendations: the review on antimicrobial resistance; 2016.

3. World Health Organization. 2019. Global tuberculosis report. World Health Organization, Geneva, Switzerland. https://www.who.int/publications/i/item/9789241565714.

4. Treatment of Tuberculosis

5. World Health Organization. 2018. The use of next-generation sequencing technologies for the detection of mutations associated with drug resistance in Mycobacterium tuberculosis complex: technical guide. World Health Organization, Geneva, Switzerland. https://apps.who.int/iris/handle/10665/274443.

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