Improved Formazan Dissolution for Bacterial MTT Assay-Reference-Cited by-同舟云学术

Improved Formazan Dissolution for Bacterial MTT Assay

Published:2021-12-22 Issue:3 Volume:9 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Benov Ludmil¹^ORCID

Affiliation:

1. Department of Biochemistry, Faculty of Medicine, Kuwait University, Kuwait City, Kuwait

Abstract

Reduction of the water-soluble tetrazolium salt 3-(4,5-dimethylthiazol)-2,5 diphenyl-tetrazolium bromide (MTT) to purple, water-insoluble formazan is commonly used for assessment of cell viability and proliferation. Spectrophotometric detection of formazan requires its solubilization.

Funder

Kuwait University

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01637-21

Reference26 articles.

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