Development of Microfluidic Chip-Based Loop-Mediated Isothermal Amplification (LAMP) Method for Detection of Carbapenemase Producing Bacteria-Reference-Cited by-同舟云学术

Development of Microfluidic Chip-Based Loop-Mediated Isothermal Amplification (LAMP) Method for Detection of Carbapenemase Producing Bacteria

Published:2022-10-26 Issue:5 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Wu Bin¹,Tong Xinxin²,Chen Bin¹,Yuan Wenchang³,Fu Mingpeng¹,Yang Xiao¹,Chen Huiling¹,Zhang Guohao⁴,Wu Guojun⁴,Xu Banglao¹^ORCID

Affiliation:

1. Department of Laboratory Medicine, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong, China

2. Department of Blood Transfusion, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong, China

3. KingMed School of Laboratory Medicine, Guangzhou Medical University, Guangzhou, Guangdong, China

4. Baicare Biotech Company, Beijing, China

Abstract

Rapid and accurate identification of CPO may reduce the genetic exchanges among bacteria and prevent further dissemination of carbapenemases to non-CPO. The current method had designed microfluidic chip-based LAMP amplification method for multiplex detection of carbapenemase genes and evaluated the detection performance of the newly method.

Funder

Guangzhou Health Science and Technology General Guidance Project

Guangzhou Planned Project of Science and Technologyy

Guangdong Medical Research Foundation

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00322-22

Reference25 articles.

1. Rapid detection of carbapenemase-producing Enterobacteriaceae from blood cultures