Characterization of a Conjugative Hybrid Plasmid Coharboring bla KPC-2 and bla IMP-4 in a Klebsiella quasipneumoniae Clinical Isolate-Reference-Cited by-同舟云学术

Characterization of a Conjugative Hybrid Plasmid Coharboring bla _KPC-2 and bla _IMP-4 in a Klebsiella quasipneumoniae Clinical Isolate

Published:2023-02-14 Issue:1 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Dong Huiyue¹,Liu Ziyi²,Wu Zhiyao¹,Zhang Tingting¹,Xia Ziwei¹,Zhao Yuxin¹,Li Yan²,Shi Jinjin¹,Wang Zhiqiang²^ORCID,Li Ruichao²³^ORCID,Qin Shangshang¹^ORCID

Affiliation:

1. School of Pharmaceutical Sciences; Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou, Henan, China

2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, People’s Republic of China

3. Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu, People’s Republic of China

Abstract

The emergence of carbapenem-resistant Enterobacterales (CRE) that coexpress serine- and metallo-carbapenemases is a severe threat to the efficacy of ceftazidime-avibactam (CZA), which has been proven to be extremely effective against KPC-producing Enterobacterales strains. Our study described the cooccurrence of KPC-2, a serine β-lactamase, and IMP-4, a metallo-β-lactamase (MBL), on a conjugative hybrid plasmid from a clinical carbapenem-resistant K. quasipneumoniae strain, and it revealed an alternative route for IncHI5 plasmid to evolve by recombining with other plasmids to form a hybrid plasmid.

Funder

National Natural Science Foundation of China

Priority Academic Program Development of Jiangsu Higher Education Institutions

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.02616-22

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