AIDmut-Seq: a Three-Step Method for Detecting Protein-DNA Binding Specificity-Reference-Cited by-同舟云学术

AIDmut-Seq: a Three-Step Method for Detecting Protein-DNA Binding Specificity

Published:2023-02-14 Issue:1 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Li Feixuan¹,Liu Xiao-Yu²,Ni Lei³,Jin Fan³^ORCID

Affiliation:

1. Hefei National Research Center for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, People’s Republic of China

2. School of Medicine, Southern University of Science and Technology, Shenzhen, People’s Republic of China

3. CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, People’s Republic of China

Abstract

Protein-DNA interactions (PDI) play a central role in gene regulatory networks (GRNs). However, current techniques for studying genome-wide PDI usually involve complex experimental procedures, which prevent their broad use by scientific researchers.

Funder

Scientific Instrument Developing Project of the Chinese Academy of Sciences

MOST | National Key Research and Development Program of China

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.03783-22

Reference41 articles.

1. Genome-wide profiles of STAT1 DNA association using chromatin immunoprecipitation and massively parallel sequencing

2. Multiplexed massively parallel SELEX for characterization of human transcription factor binding specificities

3. Mapping genome-wide transcription-factor binding sites using DAP-seq

4. ChIP–seq: advantages and challenges of a maturing technology

5. Calling Cards enable multiplexed identification of the genomic targets of DNA-binding proteins