Effect of Different Tolerable Levels of Constitutive <i>mcr-1</i> Expression on Escherichia coli-Reference-Cited by-同舟云学术

Effect of Different Tolerable Levels of Constitutive mcr-1 Expression on Escherichia coli

Published:2022-10-26 Issue:5 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Qiao Han¹²,Yu Jie¹,Wang Xiukun¹,Nie Tongying¹,Hu Xinxin¹,Yang Xinyi¹,Li Congran¹,You Xuefu¹

Affiliation:

1. Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

2. National Institutes for Food and Drug Control, Beijing, China

Abstract

The effects of relatively lower tolerable levels of mcr-1 were not evaluated thoroughly, and direct evidence for moderate resistance mediated by mcr-1 was lacking. In the present study, we made constructs carrying mcr-1 on the E. coli K12 chromosome under the control of serial constitutive promoters of different strengths and studied the effects of different tolerable levels of mcr-1 expression in vitro and in vivo .

Funder

National Natural Science Foundation of China

Chinese Academy of Medical Sciences

MOE | Fundamental Research Funds for the Central Universities

MOST | National Science and Technology Infrastructure Program

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01748-22

Reference28 articles.