Affiliation:
1. Department of Microbiology, University of Louisville School of Medicine, Louisville, Kentucky
Abstract
Bates, Janice
L. (University of Louisville School of Medicine, Louisville, Ky.)
and Pinghui V. Liu
. Complementation of lecithinase activities by closely related pseudomonads: its taxonomic implication. J. Bacteriol.
86:
585–592. 1963.—A lecithinase activity was demonstrated by a mixture of cell-free extracts from two strains of closely related pseudomonads, neither of which was able to show this activity when grown alone. One component, from B-2265 (
Pseudomonas aureofaciens
var.
nonliquefaciens
), was heatlabile, nondialyzable, and antigenic. The other component, from 22/3 (
P. fluorescens
), was relatively heat-stable, nondialyzable, and nonantigenic. Antisera produced with the component of B-2265 alone neutralized the activity of the complete enzyme made up with both components, as well as that of similar enzymes of related species, such as
P. aureofaciens, P. chlororaphis
, and
P. fluorescens
. The taxonomic implication of these findings is discussed. The suggestion is made that determination of the serological specificity of an enzyme or its antigenic subunits is a more reliable criterion for establishing relationships of bacterial strains than methods depending on the overall similarity of biochemical activities.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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