Expression of a Clostridium thermocellum endoglucanase gene in Lactobacillus plantarum-Reference-Cited by-同舟云学术

Expression of a Clostridium thermocellum endoglucanase gene in Lactobacillus plantarum

Published:1989-08 Issue:8 Volume:55 Page:2095-2097
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Bates E E¹,Gilbert H J¹,Hazlewood G P¹,Huckle J¹,Laurie J I¹,Mann S P¹

Affiliation:

1. Department of Agricultural Biochemistry and Nutrition, University of Newcastle upon Tyne, England.

Abstract

Recombinant plasmid pM25 containing the celE gene of Clostridium thermocellum, which codes for an enzymatically active endoglucanase, was transformed into Lactobacillus plantarum by electroporation. Strains harboring pM25 expressed thermostable endoglucanase, which was found predominantly in the culture medium. Two other plasmids, pGK12 and pSA3, were transformed into L. plantarum, and the stability of each plasmid was evaluated.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.55.8.2095-2097.1989

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