Formation of N,N -Dimethylglycine, Acetic Acid, and Butyric Acid from Betaine by Eubacterium limosum

Author:

Müller E.1,Fahlbusch K.1,Walther R.1,Gottschalk G.1

Affiliation:

1. Institut für Mikrobiologie der Universität Göttingen, D-3400 Göttingen, West Germany

Abstract

Two bacterial strains that grow anaerobically on betaine were isolated from enrichment cultures and identified as strains of Eubacterium limosum. In a mineral medium supplemented with yeast extract and Casitone, the doubling time of E. limosum strain 11A on betaine was 6 h at 37°C. The molar growth yield amounted to 9 g of dry cell mass per mol. Betaine was fermented in accordance with the following equation: 7 betaine + 2 CO 2 → 7 N,N -dimethylglycine + 1.5 acetate + 1.5 butyrate. E. limosum also grew on methanol and choline. The former was converted to acetate and butyrate, and the latter was converted to N,N -dimethylethanolamine, acetate, and butyrate. The conditions for the quantitative determination of N,N -dimethylglycine by capillary tube isotachophoresis have been determined.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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