Affiliation:
1. Department of Microbiology, Montana State University, Bozeman 59717.
Abstract
DNA was isolated from whole cells of Candida albicans and digested with MspI restriction enzyme. In addition to the expected large number of low-molecular-weight DNA pieces resulting from the digestion, multiple high-molecular-weight (greater than 3.0 kilobase pairs) fragments were generated by this enzyme, which cleaves DNA at CCGG sequences. Some of these fragments appeared highly repeated. An MspI fragment which was similar in size to one of the repeat elements (2.9 kilobase pairs) was cloned into the ClaI site of the plasmid vector pBR322 and replicated in a suitable Escherichia coli host strain. The candidal fragment was radiolabeled and used to probe Southern blots of DNA from several Candida species, various other fungi, and mouse and human cells. Only DNA from C. albicans and a strain of Candida stellatoidea was found to contain sequences of significant homology for hybridization. The cloned fragment may possibly be of use as a DNA probe for detection of the presence of C. albicans.
Publisher
American Society for Microbiology
Reference25 articles.
1. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells;Dagert M.;Gene,1979
2. Methods for DNA extraction from Candida albicans;Glee P. M.;Anal. Biochem.,1987
3. Application of DNA probe tests to the diagnosis of infectious disease;Kohne D. E.;Am. Clin. Prod. Rev.,1986
4. Maniatis T. E. F. Fritsch and J. Sambrook. 1982. Molecular cloning a laboratory manual. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
5. Molecular probe for identification of medically important Candida species and Torulopsis glabrata;Mason M. M.;J. Clin. Microbiol.,1987
Cited by
44 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献