Author:
Mason M M,Lasker B A,Riggsby W S
Abstract
A cloned DNA fragment from Candida albicans containing the gene for the protein actin was used to probe the molecular structure of the actin gene of several medically important yeasts (C. albicans, Candida stellatoidea, Candida tropicalis, Candida pseudotropicalis, Candida krusei, Candida parapsilosis, Candida guilliermondii, and Torulopsis glabrata). Whole-cell DNA from each species was digested with restriction endonucleases, electrophoresed on agarose gels, and transferred to nitrocellulose. Radioactively labeled C. albicans actin gene was hybridized to the DNA fragments on the nitrocellulose. The C. albicans probe produced a strong signal with all of the Candida DNAs tested, indicating considerable conservation of this gene. In addition, the actin genes of all of the species tested were found to have no internal EcoRI or SalI restriction sites. With the exception of C. guilliermondii, all of the species tested had a single internal HindIII recognition site. However, the location of flanking restriction sites was found to be species specific. For all of the enzymes tested, the locations of the flanking restriction sites in C. albicans and C. stellatoidea were identical; all of the other strains yielded fragments clearly distinct from one another. These differences provide a molecular tool for the differentiation of medically important Candida species.
Publisher
American Society for Microbiology
Reference22 articles.
1. Barnett J. A. R. W. Payne and D. Yarrow. 1983. Yeasts. Cambridge University Press. Cambridge.
2. Variability in expression of a cell surface determinant on Candida albicans as evidenced by an agglutinating monoclonal antibody;Brawner D. L.;Infect. Imtnun.,1984
3. Brown W. M. 1983. Evolution of animal mitochondrial DNA p. 62-88. Il M. Nei and R. K. Koehn (ed.) Evolution of genes and proteins. Sinauer Associates Sunderland Mass.
4. Davis R. W. D. Botstein and J. R. Roth. 1980. Advanced bacterial genetics. Cold Spring Harbor Laboratory Cold Spring Harbor. N.Y.
5. Gabriel-Bruneau S. M. and R. M. F. Guinet. 1984. Antigenic 566 NOTES
Cited by
77 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献