Enrichment Double-Antibody Sandwich Indirect Enzyme-Linked Immunosorbent Assay That Uses a Specific Monoclonal Antibody for Sensitive Detection of Ralstonia solanacearum in Asymptomatic Potato Tubers-Reference-Cited by-同舟云学术

Enrichment Double-Antibody Sandwich Indirect Enzyme-Linked Immunosorbent Assay That Uses a Specific Monoclonal Antibody for Sensitive Detection of Ralstonia solanacearum in Asymptomatic Potato Tubers

Published:2002-07 Issue:7 Volume:68 Page:3634-3638
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Caruso Paola¹,Gorris María Teresa¹,Cambra Mariano¹,Palomo José Luis²,Collar Jesús³,López María M.¹

Affiliation:

1. Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial, 46113 Moncada, Valencia

2. Centro Regional de Diagnóstico, Apartado 61, 37080 Salamanca

3. Laboratorio Agrario e Fitopatolóxico de Galicia, 15318 Mabegondo, La Coruña, Spain

Abstract

ABSTRACT Sensitive and specific routine detection of Ralstonia solanacearum in symptomless potato tubers was achieved by efficient enrichment followed by a reliable double-antibody sandwich indirect enzyme-linked immunosorbent assay based on the specific monoclonal antibody 8B-IVIA. This monoclonal antibody reacted with 168 typical R. solanacearum strains and did not recognize 174 other pathogenic or unidentified bacteria isolated from potato. The optimized protocol included an initial enrichment step consisting of shaking the samples in modified Wilbrink broth for 72 h at 29°C. This step enabled specific detection by the enzyme-linked immunosorbent assay of 1 to 10 CFU of R. solanacearum per ml of initial potato extract. Analysis of 233 commercial potato lots by this method provided results that coincided with the results of conventional methods.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.68.7.3634-3638.2002

Reference27 articles.

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