Enrichment Double-Antibody Sandwich Indirect Enzyme-Linked Immunosorbent Assay That Uses a Specific Monoclonal Antibody for Sensitive Detection of Ralstonia solanacearum in Asymptomatic Potato Tubers-Reference-Cited by-同舟云学术

Enrichment Double-Antibody Sandwich Indirect Enzyme-Linked Immunosorbent Assay That Uses a Specific Monoclonal Antibody for Sensitive Detection of Ralstonia solanacearum in Asymptomatic Potato Tubers

Published:2002-07 Issue:7 Volume:68 Page:3634-3638
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Caruso Paola¹,Gorris María Teresa¹,Cambra Mariano¹,Palomo José Luis²,Collar Jesús³,López María M.¹

Affiliation:

1. Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial, 46113 Moncada, Valencia

2. Centro Regional de Diagnóstico, Apartado 61, 37080 Salamanca

3. Laboratorio Agrario e Fitopatolóxico de Galicia, 15318 Mabegondo, La Coruña, Spain

Abstract

ABSTRACT Sensitive and specific routine detection of Ralstonia solanacearum in symptomless potato tubers was achieved by efficient enrichment followed by a reliable double-antibody sandwich indirect enzyme-linked immunosorbent assay based on the specific monoclonal antibody 8B-IVIA. This monoclonal antibody reacted with 168 typical R. solanacearum strains and did not recognize 174 other pathogenic or unidentified bacteria isolated from potato. The optimized protocol included an initial enrichment step consisting of shaking the samples in modified Wilbrink broth for 72 h at 29°C. This step enabled specific detection by the enzyme-linked immunosorbent assay of 1 to 10 CFU of R. solanacearum per ml of initial potato extract. Analysis of 233 commercial potato lots by this method provided results that coincided with the results of conventional methods.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.68.7.3634-3638.2002

Reference27 articles.

1. Alarcón, B., M. M. López, M. Cambra, M. T. Gorris, and J. Guerri. 1990. Differentation of Erwinia carotovora subsp. carotovora and Erwinia carotovora subsp. atroseptica isolated from potato by Western blot and subsequent indirect ELISA. J. Appl. Bacteriol.69:17-20.

2. Alarcón, B., M. T. Gorris, M. Cambra, and M. M. López. 1995. Serological characterization of potato isolates of Erwinia carotovora subsp. atroseptica and subsp. carotovora using polyclonal and monoclonal antibodies. J. Appl. Bacteriol.79:592-602.

3. Alvarez A. M. J. I. Berestecky J. I. Stiles S. A. Ferreira and A. A. Benedict. 1992. Serological and molecular approaches to identification of Pseudomonas solanacearum strains from Heliconia p. 62-69. In G. L. Hartman and A. C. Hayward (ed.) Bacterial wilt. Proceedings of the International Conference. ACIAR Proceedings no. 31. Australian Centre for International Agricultural Research Canberra Australia.

4. Anonymous. 1998. Ralstonia solanacearum race 3, p. 276. In I. M. Smith and L. M. F. Charles (ed.), Distribution maps of quarantine pests for Europe. CAB International, Wallingford, United Kingdom.

5. Caruso P. P. Llop J. L. Palomo P. García C. Morente and M. M. López. 1998. Evaluation of methods for detection of potato seed contamination by Ralstonia solanacearum p. 128-132. In P. Prior C. Allen and J. Elphinstone (ed.) Bacterial wilt disease. Molecular and ecological aspects. Springer Verlag Heidelberg Germany.

Cited by 42 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Assessment and detection of biotic and abiotic stresses in field crops through remote and proximal sensing techniques—evidence from earlier findings;Arabian Journal of Geosciences;2024-05-24

2. Conservative gene recognition-promoted electrochemical strategy for sensitive detection of Ralstonia Solanacearum phylotype i tobacco strains in China;Sensors and Actuators B: Chemical;2024-03

3. Recent advances in immuno-based methods for the detection of Ralstonia solanacearum;Journal of Microbiological Methods;2024-02

4. Next‐generation methods for early disease detection in crops;Pest Management Science;2023-09-20

5. Loop-mediated isothermal amplification (LAMP)/Cas12a assay for detection of Ralstonia solanacearum in tomato;Frontiers in Bioengineering and Biotechnology;2023-05-22