Next‐generation methods for early disease detection in crops

Author:

Trippa Daniela1,Scalenghe Riccardo1ORCID,Basso Marcos Fernando2,Panno Stefano1,Davino Salvatore1,Morone Chiara3,Giovino Antonio4,Oufensou Safa5ORCID,Luchi Nicola6,Yousefi Sanaz7,Martinelli Federico26ORCID

Affiliation:

1. Dipartimento di Scienze Agrarie Alimentari e Forestali Università degli Studi di Palermo Palermo Italy

2. Department of Biology University of Florence Florence Italy

3. Regione Piemonte ‐ Phytosanitary Division Torino Italy

4. Council for Agricultural Research and Economics (CREA)—Research Centre for Plant Protection and Certification (CREA‐DC) Palermo Italy

5. Dipartimento di Agraria Università degli Studi di Sassari Sassari Italy

6. National Research Council, Institute for Sustainable Plant Protection, (CNR‐IPSP) Florence Italy

7. Department of Horticultural Science Bu‐Ali Sina University Hamedan Iran

Abstract

AbstractPlant pathogens are commonly identified in the field by the typical disease symptoms that they can cause. The efficient early detection and identification of pathogens are essential procedures to adopt effective management practices that reduce or prevent their spread in order to mitigate the negative impacts of the disease. In this review, the traditional and innovative methods for early detection of the plant pathogens highlighting their major advantages and limitations are presented and discussed. Traditional techniques of diagnosis used for plant pathogen identification are focused typically on the DNA, RNA (when molecular methods), and proteins or peptides (when serological methods) of the pathogens. Serological methods based on mainly enzyme‐linked immunosorbent assay (ELISA) are the most common method used for pathogen detection due to their high‐throughput potential and low cost. This technique is not particularly reliable and sufficiently sensitive for many pathogens detection during the asymptomatic stage of infection. For non‐cultivable pathogens in the laboratory, nucleic acid‐based technology is the best choice for consistent pathogen detection or identification. Lateral flow systems are innovative tools that allow fast and accurate results even in field conditions, but they have sensitivity issues to be overcome. PCR assays performed on last‐generation portable thermocyclers may provide rapid detection results in situ. The advent of portable instruments can speed pathogen detection, reduce commercial costs, and potentially revolutionize plant pathology. This review provides information on current methodologies and procedures for the effective detection of different plant pathogens. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Publisher

Wiley

Subject

Insect Science,Agronomy and Crop Science,General Medicine

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