Association of a 13.6-Megadalton Plasmid in Pediococcus pentosaceus with Bacteriocin Activity

Author:

Daeschel Mark A.1,Klaenhammer Todd R.1

Affiliation:

1. Food Fermentation Laboratory, Agricultural Research Service, U.S. Department of Agriculture, and North Carolina Agricultural Research Service, Department of Food Science, North Carolina State University, 2 Raleigh, North Carolina 27695-7624

Abstract

Pediococcus pentosaceus FBB61 and L7230, isolated from cucumber fermentations, produced a bacteriocin, designated pediocin A, which had identical activity spectra against species of Pediococcus, Clostridium, Staphylococcus , and Streptococcus . Both strains possessed a 13.6-megadalton plasmid (pMD136). Plasmid curing experiments suggested that both bacteriocin immunity and production determinants were encoded by pMD136. Use of pediocin-producing strains in food fermentations is discussed. Images

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference14 articles.

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3. A medium for cultivation of lactobacilli;DeMan J. C.;J. Appl. Bacteriol.,1960

4. Selection in chemostats;Dykhuizen D. E.;Microbiol. Rev.,1983

5. Pure culture fermentation of brined cucumbers;Etchells J. L.;Appl. Microbiol.,1964

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