Affiliation:
1. Laboratory of Industrial Microbiology and Biochemistry, University of Leuven, B-3030 Leuven, Belgium
Abstract
The extracellular amylolytic system of
Filobasidium capsuligenum
consisted of an α-amylase (1,4-α-
d
-glucan glucanhydrolase, EC 3.2.1.1) and two forms of glucoamylase (1,4-α-
d
-glucan glucohydrolase, EC 3.2.1.3). The enzymes were purified by ammonium sulfate fractionation, repeated ion-exchange chromatography (DEAE-Sephadex A-50), and gel filtration (Sephadex G-25, Sephadex G-100 sf). α-Amylase had an optimum pH of 5.6 and an optimum temperature of 50�C but was rapidly inactivated at higher temperature. The molecular weight was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 64,000. An acarbose concentration of 20 μg/ml was required for 50% inhibition of the α-amylase. Both glucoamylases are glycoproteins of identical molecular weight (60,000) and produce only glucose by exohydrolysis. The debranching activity of the glucoamylases was evidenced with substrates containing α-1,6 linkages. The pH optima were 5.0 to 5.6 for glucoamylase I and 4.8 to 5.3 for glucoamylase II. Glucoamylase I had a higher optimum temperature (55�C) than glucoamylase II (50�C) and was also more resistant to thermal inactivation. Only low acarbose concentrations (<0.1 μg/ml) were required to reduce the activity of the glucoamylases by 50%.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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