Susceptibility ofHaemophilus influenzaeto Ampicillin as Determined by Use of a Modified, One-Minute Beta-Lactamase Test

Author:

Escamilla Joel1

Affiliation:

1. Navy Environmental and Preventive Medicine Unit No. 2, Norfolk, Virginia 23511

Abstract

Fifty-seven cultures ofHaemophilus influenzaewere tested for production of beta-lactamase by use of a modified, 1-min test. Only the 24 cultures that were ampicillin resistant, whether type b or not, yielded positive results for the enzyme; all ampicillin-susceptible isolates were uniformly negative. The beta-lactamase procedure is simple to perform and requires only commonly available reagents.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference9 articles.

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2. Beta-lactamase activity in ampicillin-resistant Haemophilus influenzae;Farrar W. E.;Antimicrob. Agents Chemother.,1974

3. Haemophilus influenzae type B resistant to ampicillin. A report of two cases;Kahn W.;J. Am. Med. Assoc. 229-298-301.,1974

4. Antimicrobial susceptibility of Haemophilus influenzae;McLinn S. E.;Pediatrics,1970

5. Ampicillin resistant Haemophilus influenzae type B possessing a TEM-type ,-lactamase but little permeability barrier to ampiciflin;Maderios A. A.;Lancet,1975

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