The ygaVP Genes of Escherichia coli Form a Tributyltin-Inducible Operon

Author:

Gueuné Hervé12,Durand Marie-José1,Thouand Gérald1,DuBow Michael S.3

Affiliation:

1. Université de Nantes, CNRS UMR 6144, GEPEA, ERT CBAC, Département Génie Biologique, 18 Bd. Gaston Defferre, 85035 La Roche sur Yon, France

2. Biolumine SA, Site Universitaire de la Courtaisière, Département Génie Biologique,18 Bd. Gaston Defferre, 85035 La Roche sur Yon, France

3. Université Paris-Sud 11, CNRS UMR 8621, Institut de Génétique et Microbiologie, Bâtiment 409, 91405 Orsay, France

Abstract

ABSTRACT A tributyltin (TBT) luxAB transcriptional fusion in Escherichia coli revealed that a TBT-activated promoter is located upstream of two cotranscribed orphan genes, ygaV and ygaP . We demonstrate that transcription from the promoter upstream of ygaVP is constitutive in a ygaVP mutant, suggesting that YgaV is an autoregulated, TBT-inducible repressor.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference19 articles.

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3. Briscoe, S. F., C. Diorio, and M. S. DuBow. 1996. Luminescent biosensors for the detection of tributyltin and dimethyl sulfoxide and the elucidation of their mechanisms of toxicity, p. 645-655. In M. Moo-Young, W. A. Anderson, and A. M. Chakrabarty (ed.), Environmental biotechnology, principles and applications. Kluwer Academic Press, Dordrecht, The Netherlands.

4. Busenlehner, L. S., M. A. Pennella, and D. P. Giedroc. 2003. The SmtB/ArsR family of metalloregulatory transcriptional repressors: structural insights into prokaryotic metal resistance. FEMS Microbiol. Rev.27:131-143.

5. Développement d'un biocapteur bactérien bioluminescent multicanal pour la détection de polluants dans l'environnement. 2006

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