Type VII Secretion Substrates of Pathogenic Mycobacteria Are Processed by a Surface Protease-Reference-Cited by-同舟云学术

Type VII Secretion Substrates of Pathogenic Mycobacteria Are Processed by a Surface Protease

Published:2019-10-29 Issue:5 Volume:10 Page:
ISSN:2161-2129
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Burggraaf Maroeska J.¹,Speer Alexander²,Meijers Aniek S.²,Ummels Roy²,van der Sar Astrid M.²,Korotkov Konstantin V.³^ORCID,Bitter Wilbert²⁴,Kuijl Coenraad²^ORCID

Affiliation:

1. Amsterdam UMC, Vrije Universiteit Amsterdam, Medical Microbiology and Infection Control, Cancer Center Amsterdam, Amsterdam, Netherlands

2. Amsterdam UMC, Vrije Universiteit Amsterdam, Medical Microbiology and Infection Control, Amsterdam Institute of Infection & Immunity, Amsterdam, Netherlands

3. Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, Kentucky, USA

4. Molecular Microbiology, Vrije Universiteit Amsterdam, Amsterdam, Netherlands

Abstract

Aspartic proteases are common in eukaryotes and retroviruses but are relatively rare among bacteria (N. D. Rawlings and A. Bateman, BMC Genomics 10:437, 2009, https://doi.org/10.1186/1471-2164-10-437 ). In contrast to eukaryotic aspartic proteases, bacterial aspartic proteases are generally located in the cytoplasm. We have identified a surface-associated mycobacterial aspartic protease, PecA, which cleaves itself and many other type VII secretion substrates of the PE_PGRS family. PecA is present in most pathogenic mycobacterial species, including M. tuberculosis . In addition, pathogenicity of M. marinum is reduced in the ΔpecA mutant, indicating that PecA contributes to virulence.

Funder

Cancer Center Amsterdam

NWO VENI

National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mBio.01951-19

Reference40 articles.

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5. Loss of RD1 contributed to the attenuation of the live tuberculosis vaccines Mycobacterium bovis BCG and Mycobacterium microti

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