Preparation, Sensitivity, and Specificity of Limulus Lysate for Endotoxin Assay-Reference-Cited by-同舟云学术

Preparation, Sensitivity, and Specificity of Limulus Lysate for Endotoxin Assay

Published:1973-07 Issue:1 Volume:26 Page:43-48
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Jorgensen James H.¹²,Smith Rodney F.¹²

Affiliation:

1. Division of Microbiology, Shriners Burns Institute, Galveston, Texas 77550

2. Department of Microbiology, the University of Texas Medical Branch, Galveston, Texas 77550

Abstract

Limulus amoebocyte lysate was prepared from a total of 180 crabs during 1971 and 1972 by using a slightly modified lysate preparation procedure. Marked variability of lysate potency was noted both years. In addition, lysate quality appeared diminished in 1972 as compared with 1971. Different lysate batches were evaluated for potency by using a variety of endotoxin preparations. Variations in batch potencies were observed, but little variation in reactivity among different endotoxin preparations was noted. Use of potent lysate batches allowed detection of endotoxin concentrations as low as 100 pg/ml. No endotoxinlike activity was observed from 11 different strains of yeast by use of the Limulus assay.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.26.1.43-48.1973

Reference13 articles.

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2. Quantitative comparison of in-vitro and in-vivo methods for the detection of endotoxin;Cooper J. F.;J. Lab. Clin. Med.,1971

3. The Limulus test for endotoxin (pyrogen) in radiopharmaceuticals and biologicals;Cooper J. F.;Bull. Parenter. Drug Ass.,1972

4. Role of intraintestinal endotoxin in death from peritonitis;Cuevas P.;Surg. Gynecol. Obstet.,1972

5. Biological and chemical characterization of toxic substances from Candida albicans;Cutler J. E.;Appl. Microbiol.,1972

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1. Purification and characterization of an endotoxin-binding protein with protease inhibitory activity from Limulus amebocytes.;Journal of Biological Chemistry;1991-11

2. Studies on the sensitivity and specificity of the Limulus amebocyte lysate test and rabbit pyrogen assays;Applied and Environmental Microbiology;1985-12

3. Presence of microorganisms in hemolymph of the horseshoe crab Limulus polyphemus;Applied and Environmental Microbiology;1985-03

4. Rapid detection of significant bacteriuria by use of an automated Limulus amoebocyte lysate assay;Journal of Clinical Microbiology;1982-09

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