Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis

Author:

Abd Algaffar S. O.12ORCID,Verbon A.2,van de Sande W. W. J.2ORCID,Khalid S. A.1ORCID

Affiliation:

1. Faculty of Pharmacy, University of Science & Technology, Omdurman, Sudan

2. Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands

Abstract

We present an in vitro susceptibility assay for Madurella mycetomatis hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference15 articles.

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5. Testing of the In Vitro Susceptibilities of Madurella mycetomatis to Six Antifungal Agents by Using the Sensititre System in Comparison with a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5- [(Phenylamino)Carbonyl]-2 H -Tetrazolium Hydroxide (XTT) Assay and a Modified NCCLS Method

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