Flavin Mononucleotide-Based Fluorescent Protein as an Oxygen-Independent Reporter in Candida albicans and Saccharomyces cerevisiae-Reference-Cited by-同舟云学术

Flavin Mononucleotide-Based Fluorescent Protein as an Oxygen-Independent Reporter in Candida albicans and Saccharomyces cerevisiae

Published:2009-06 Issue:6 Volume:8 Page:913-915
ISSN:1535-9778
Container-title:Eukaryotic Cell
language:en
Short-container-title:Eukaryot Cell

Author:

Tielker D.¹,Eichhof I.¹,Jaeger K.-E.²,Ernst J. F.¹

Affiliation:

1. Institut für Mikrobiologie

2. Institut für Molekulare Enzymtechnologie, Forschungszentrum Jülich, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany

Abstract

ABSTRACT Hypoxia is encountered frequently by pathogenic and apathogenic fungi. A codon-adapted gene encoding flavin mononucleotide-based fluorescent protein (Ca FbFP ) was expressed in Candida albicans and Saccharomyces cerevisiae . Both species produced CaFbFP and fluoresced even during hypoxia, suggesting that oxygen-independent CaFbFP is a useful, novel tool for monitoring hypoxic gene expression in fungi.

Publisher

American Society for Microbiology

Subject

Molecular Biology,General Medicine,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/EC.00394-08

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