Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing-Reference-Cited by-同舟云学术

Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing

Published:2021-06-03 Issue:22 Volume:10 Page:
ISSN:2576-098X
Container-title:Microbiology Resource Announcements
language:en
Short-container-title:Microbiol Resour Announc

Author:

Woudstra Cedric¹^ORCID,Mäklin Tommi²,Derman Yagmur¹,Bano Luca³,Skarin Hanna⁴,Mazuet Christelle⁵,Honkela Antti⁶,Lindström Miia¹

Affiliation:

1. Department of Food Hygiene and Environmental Health, University of Helsinki, Helsinki, Finland

2. Department of Mathematics and Statistics, University of Helsinki, Helsinki, Finland

3. Microbiology Laboratory, Istituto Zooprofiilattico Sperimentale delle Venezie, Villorba, Italy

4. National Veterinary Institute, Uppsala, Sweden

5. National Reference Center for Anaerobic Bacteria and Botulism, Institut Pasteur, Paris, France

6. Department of Computer Science, University of Helsinki, Helsinki, Finland

Abstract

Clostridium botulinum group III is the anaerobic Gram-positive bacteria producing the deadly neurotoxin responsible for animal botulism. Here, we used long-read sequencing to produce four complete genomes from Clostridium botulinum group III neurotoxin types C, D, C/D, and D/C. The protocol to obtain high-molecular-weight DNA from C. botulinum group III is described.

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MRA.01364-20

Reference12 articles.

1. Botulism

2. Botulismus beim Rind

3. Investigation ofClostridium botulinumin commercial poultry farms in France between 2011 and 2013

4. Evidence for a natural humoral response in dairy cattle affected by persistent botulism sustained by non-chimeric type C strains

5. Plasmidome Interchange between Clostridium botulinum, Clostridium novyi and Clostridium haemolyticum Converts Strains of Independent Lineages into Distinctly Different Pathogens

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