Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing

Author:

Woudstra Cedric1ORCID,Mäklin Tommi2,Derman Yagmur1,Bano Luca3,Skarin Hanna4,Mazuet Christelle5,Honkela Antti6,Lindström Miia1

Affiliation:

1. Department of Food Hygiene and Environmental Health, University of Helsinki, Helsinki, Finland

2. Department of Mathematics and Statistics, University of Helsinki, Helsinki, Finland

3. Microbiology Laboratory, Istituto Zooprofiilattico Sperimentale delle Venezie, Villorba, Italy

4. National Veterinary Institute, Uppsala, Sweden

5. National Reference Center for Anaerobic Bacteria and Botulism, Institut Pasteur, Paris, France

6. Department of Computer Science, University of Helsinki, Helsinki, Finland

Abstract

Clostridium botulinum group III is the anaerobic Gram-positive bacteria producing the deadly neurotoxin responsible for animal botulism. Here, we used long-read sequencing to produce four complete genomes from Clostridium botulinum group III neurotoxin types C, D, C/D, and D/C. The protocol to obtain high-molecular-weight DNA from C. botulinum group III is described.

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

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