Invader Plus Method Detects Herpes Simplex Virus in Cerebrospinal Fluid and Simultaneously Differentiates Types 1 and 2-Reference-Cited by-同舟云学术

Invader Plus Method Detects Herpes Simplex Virus in Cerebrospinal Fluid and Simultaneously Differentiates Types 1 and 2

Published:2006-09 Issue:9 Volume:44 Page:3443-3447
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Allawi Hatim T.¹,Li Haijing²,Sander Tamara¹,Aslanukov Azamat¹,Lyamichev Victor I.¹,Blackman Amondrea²,Elagin Slava¹,Tang Yi-Wei²

Affiliation:

1. Third Wave Technologies, Inc., Madison, Wisconsin 53719

2. Vanderbilt University Medical Center, Nashville, Tennessee 37232-5310

Abstract

ABSTRACT We report here on the development and validation of a prototype Invader Plus method for the qualitative detection of herpes simplex virus types 1 and 2 in cerebrospinal fluid (CSF). The method combines PCR and Invader techniques in a single, closed-tube, continuous-reaction format that gives an analytical sensitivity of approximately 10 copies per reaction. The clinical sensitivity and specificity were 100.0% and 98.6%, respectively, when the results of the method were validated against the results obtained with a PCR colorimetric microtiter plate system by use of clinical CSF specimens.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01175-06

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