Complete Genome Sequences of Lambdoid Phages 21, 434, and 434B and Several Lambda Hybrids

Author:

Feiss Mike1ORCID,Adhya Sankar2,Georgopoulos Costa3,Hendrix Roger W.4,Hatfull Graham F.4ORCID,Gilcrease Eddie B.5,Casjens Sherwood R.67ORCID,Ramsey Jolene89ORCID,Young Ry89ORCID

Affiliation:

1. Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA

2. Laboratory of Molecular Biology, Center for Cancer Research, The National Cancer Institute, Bethesda, Maryland, USA

3. Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA

4. Department of Biological Sciences, Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

5. Department of Civil and Environmental Engineering, University of Utah, Salt Lake City, Utah, USA

6. Division of Microbiology and Immunology, Pathology Department, University of Utah School of Medicine, Salt Lake City, Utah, USA

7. School of Biological Sciences, University of Utah, Salt Lake City, Utah, USA

8. Center for Phage Technology, Texas A&M University, College Station, Texas, USA

9. Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA

Abstract

Recombinational hybrids between phage λ and its relatives were instrumental in the beginnings of molecular biology. Here, we report the complete genome sequences of lambdoid phages 21 and 434 and three of their λ hybrids. In addition, we describe 434B, where the entire lysis gene region was replaced by cryptic prophage sequences.

Funder

HHS | National Institutes of Health

National Science Foundation

HHS | NIH | National Cancer Institute

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

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