Recombinant Protein-Based Assays for Detection of Antibodies to Severe Acute Respiratory Syndrome Coronavirus Spike and Nucleocapsid Proteins-Reference-Cited by-同舟云学术

Recombinant Protein-Based Assays for Detection of Antibodies to Severe Acute Respiratory Syndrome Coronavirus Spike and Nucleocapsid Proteins

Published:2007-03 Issue:3 Volume:14 Page:331-333
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Haynes Lia M.¹²³⁴⁵,Miao Congrong¹²³⁴⁵,Harcourt Jennifer L.¹²³⁴⁵,Montgomery Joel M.¹²³⁴⁵,Le Mai Quynh¹²³⁴⁵,Dryga Sergey A.¹²³⁴⁵,Kamrud Kurt I.¹²³⁴⁵,Rivers Bryan¹²³⁴⁵,Babcock Gregory J.¹²³⁴⁵,Oliver Jennifer Betts¹²³⁴⁵,Comer James A.¹²³⁴⁵,Reynolds Mary¹²³⁴⁵,Uyeki Timothy M.¹²³⁴⁵,Bausch Daniel¹²³⁴⁵,Ksiazek Thomas¹²³⁴⁵,Thomas William¹²³⁴⁵,Alterson Harold¹²³⁴⁵,Smith Jonathan¹²³⁴⁵,Ambrosino Donna M.¹²³⁴⁵,Anderson Larry J.¹²³⁴⁵

Affiliation:

1. National Centers for Immunization and Respiratory Diseases, Division of Viral Diseases, Respiratory and Gastroenteritis Viruses Branch

2. Division of Viral and Rickettsial Diseases, Special Pathogens Branch

3. Poxvirus Activity

4. Influenza Division, Centers for Disease Control and Prevention (CDC), 1600 Clifton Rd. NE, Atlanta, Georgia

5. AlphaVax, Inc., Research Triangle Park, North Carolina

Abstract

ABSTRACT Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00351-06

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