Development and Evaluation of a Line Probe Assay for Rapid Identification of pncA Mutations in Pyrazinamide-Resistant Mycobacterium tuberculosis Strains-Reference-Cited by-同舟云学术

Development and Evaluation of a Line Probe Assay for Rapid Identification of pncA Mutations in Pyrazinamide-Resistant Mycobacterium tuberculosis Strains

Published:2007-09 Issue:9 Volume:45 Page:2802-2807
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Sekiguchi Jun-Ichiro¹,Nakamura Tomohiko²,Miyoshi-Akiyama Tohru¹,Kirikae Fumiko¹,Kobayashi Intetsu³,Augustynowicz-Kopeć Ewa⁴,Zwolska Zofia⁴,Morita Koji⁵,Suetake Toshinori²,Yoshida Hiroshi²,Kato Seiya⁶,Mori Toru⁷,Kirikae Teruo¹

Affiliation:

1. Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan

2. Third Department, Research and Development Laboratory, Nipro Corporation, 3023 Noji, Kusatsu, Shiga 525-0055, Japan

3. Mitsubishi Chemical Medience Corporation, 3-30-1 Shimura, Itabashi, Tokyo 174-8555, Japan

4. National Research Institute of Tuberculosis and Lung Diseases, Plocka St. 26, Warsaw 01-138, Poland

5. Department of Microbiology, Kyorin University School of Health Sciences, 476 Miyashita, Hachioji, Tokyo 192-8508, Japan

6. Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Matsuyama 3-1-24, Kiyose, Tokyo 204-8533, Japan

7. Leprosy Research Center, National Institute of Infectious Diseases, Aoba 4-2-1, Higashimurayama, Tokyo 189-0002, Japan

Abstract

ABSTRACT Resistance of Mycobacterium tuberculosis to pyrazinamide (PZA) derives mainly from mutations in the pncA gene. We developed a reverse hybridization-based line probe assay with oligonucleotide probes designed to detect mutations in pncA . The detection of PZA resistance was evaluated in 258 clinical isolates of M. tuberculosis . The sensitivity and specificity of PZA resistance obtained by this new assay were both 100%, consistent with the results of conventional PZA susceptibility testing. This assay can be used with sputa from tuberculosis patients. It appears to be reliable and widely applicable and, given its simplicity and rapid performance, will be a valuable tool for diagnostic use.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00352-07

Reference22 articles.

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