Detection of Rifampin-Resistant Mycobacterium tuberculosis in Sputa by Nested PCR-Linked Single-Strand Conformation Polymorphism and DNA Sequencing-Reference-Cited by-同舟云学术

Detection of Rifampin-Resistant Mycobacterium tuberculosis in Sputa by Nested PCR-Linked Single-Strand Conformation Polymorphism and DNA Sequencing

Published:2001-07 Issue:7 Volume:39 Page:2610-2617
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kim Bum-Joon¹,Lee Keun-Hwa²,Park Bo-Na²,Kim Seo-Jeong³,Park Eun-Mi⁴,Park Young-Gil⁴,Bai Gil-Han⁴,Kim Sang-Jae⁴,Kook Yoon-Hoh²

Affiliation:

1. Department of Microbiology, Cheju National University College of Medicine, Cheju 690-756,1

2. Department of Microbiology and Institute of Endemic Diseases, Medical Research Center, Seoul National University College of Medicine, and Clinical Research Institute, Seoul National University Hospital, Seoul 110-799,2

3. Department of Pediatrics, Pundang CHA General Hospital, Pochun CHA Medical School, Kyonggi Sungnam 463-670,3 and

4. The Korean Institute of Tuberculosis, the Korean National Tuberculosis Association, Seoul 137-140,4 Korea

Abstract

ABSTRACT Either PCR-mediated single strand conformation polymorphism (SSCP) analysis or DNA sequencing of rpoB DNA (157 bp) can be used as a rapid screening method for the detection of mutations related to the rifampin resistance of Mycobacterium tuberculosis . However, due to the nonspecific amplification of rpoB DNA from nontuberculous mycobacteria these methods cannot be directly applied to clinical specimens such as sputa. We developed a nested PCR method that can specifically amplify the rpoB DNA of M. tuberculosis on the basis of rpoB DNA sequences of 44 mycobacteria. Nested PCR-linked SSCP analysis and the DNA sequencing method were applied directly in order to detect M. tuberculosis and determine its rifampin susceptibility in 56 sputa. The results obtained by nested PCR-SSCP and DNA sequencing were concordant with those of conventional drug susceptibility testing and DNA sequencing performed with culture isolates.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.7.2610-2617.2001

Reference23 articles.

1. Tuberculosis in patients with immunodeficiency virus infection;Barnes P.;N. Engl. J. Med.,1991

2. Rapid and simple method for purification of nucleic acids

3. A controlled trial of six months chemotherapy in pulmonary tuberculosis;British Thoracic Association;Am. Rev. Respir. Dis.,1982

4. Rapid detection of rifampin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay;De Beenhouwer H.;Tuber. Lung Dis.,1995

5. Des Prez R. M. Heim C. R. Mycobacterium tuberculosis Principles and practice of infectious diseases 2nd ed. Mandell G. L. Douglas R. G. Bennett J. E. 1990 1877 1906 Churchill Livingstone New York N.Y

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