Rapid Genotypic Detection of Rifampin- and Isoniazid-Resistant Mycobacterium tuberculosis Directly in Clinical Specimens

Author:

Bang Didi1,Bengård Andersen Åse2,Thomsen Vibeke Østergaard1

Affiliation:

1. International Reference Laboratory of Mycobacteriology, Statens Serum Institut, National Institute for Prevention and Control of Infectious Diseases and Congenital Disorders, Artillerivej 5, 2300 Copenhagen, Denmark

2. Department of Infectious Diseases, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark

Abstract

ABSTRACT A multiplex PCR DNA strip assay (Genotype MTBDR) designed to detect rifampin ( rpoB ) and high-level isoniazid ( katG ) resistance mutations in Mycobacterium tuberculosis isolates was optimized for clinical specimens. Successful genotypic results were achieved with 36 of 38 (95%) smear-positive respiratory specimens, allowing rapid therapeutic adjustments in transmittable drug-resistant tuberculosis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference24 articles.

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