Enhancing Effect of Feces on Isolation of Salmonellae From Selenite Broth

Abstract

The addition of feces to selenite broth significantly enhanced the ability of this medium to select for salmonellae in an environment initially containing overwhelming numbers of coliform bacteria. Either heat-sterilized or Seitz-filtered feces produced this effect. In most experiments, the selectivity of selenite broth was unaffected by unsterile feces. Human blood and plasma markedly reduced selenite efficiency. In a base medium supporting both coliform and Shigella growth, heat-sterilized feces imposed a measure of selectivity for Shigella .